Analysis of fluorescently labeled glycosphingolipid-derived oligosaccharides following ceramide glycanase digestion and anthranilic acid labeling.

@article{Neville2004AnalysisOF,
  title={Analysis of fluorescently labeled glycosphingolipid-derived oligosaccharides following ceramide glycanase digestion and anthranilic acid labeling.},
  author={David C. A. Neville and Virginie Coquard and David A Priestman and Danielle J M te Vruchte and Daniel J. Sillence and Raymond A. Dwek and Frances M. Platt and Terry D. Butters},
  journal={Analytical biochemistry},
  year={2004},
  volume={331 2},
  pages={
          275-82
        }
}
Interest in cellular glycosphingolipid (GSL) function has necessitated the development of a rapid and sensitive method to both analyze and characterize the full complement of structures present in various cells and tissues. An optimized method to characterize oligosaccharides released from glycosphingolipids following ceramide glycanase digestion has been developed. The procedure uses the fluorescent compound anthranilic acid (2-aminobenzoic acid; 2-AA) to label oligosaccharides prior to… CONTINUE READING

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