Analysis of flavonoids in honey by HPLC coupled with coulometric electrode array detection and electrospray ionization mass spectrometry

@article{Petrus2011AnalysisOF,
  title={Analysis of flavonoids in honey by HPLC coupled with coulometric electrode array detection and electrospray ionization mass spectrometry},
  author={Karoline Petrus and Heidi Schwartz and Gerhard Sontag},
  journal={Analytical and Bioanalytical Chemistry},
  year={2011},
  volume={400},
  pages={2555-2563}
}
AbstractThe analysis of flavonoids in unifloral honeys by high-performance liquid chromatography (HPLC) coupled with coulometric electrode array detection (CEAD) is described. The compounds were extracted by a nonionic polymeric resin (Amberlite XAD-2) and then separated on a reversed phase column using gradient elution. Quercetin, naringenin, hesperetin, luteolin, kaempferol, isorhamnetin, and galangin were detected in a coulometric electrode array detection system between +300 and +800 mV… 

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References

SHOWING 1-10 OF 44 REFERENCES

Determination of antioxidant flavonoids in Sudanese honey samples by solid phase extraction and high performance liquid chromatography.

Flavonoids were extracted by solid phase extraction (SPE) from seven floral honey samples of different botanical origin from different regions of Sudan. The flavonoids were determined by high

Confirmation of phenolic acids and flavonoids in honeys by UPLC-MS

Certain phenolic acids and flavonoids are described in the literature as marker substances for several unifloral honeys. As not all authors utilised the same methods for extraction and determination,

An HPLc technique for flavonoid analysis in honey

A new technique for the analysis of flavonoids in honey has been developed. This uses filtration of honey through Amberlite-XAD-2 and purification of the flavonoid fraction by Sephadex LH-20. The

Identification of phenolic compound in manuka honey as specific superoxide anion radical scavenger using electron spin resonance (ESR) and liquid chromatography with coulometric array detection

Apitherapy has become the focus of attention as a form of folk and preventive medicine for treating certain conditions and diseases as well as promoting overall health and well-being. In apitherapy,

Separation of flavonoids and phenolic acids from propolis by capillary zone electrophoresis

  • N. Volpi
  • Chemistry, Medicine
    Electrophoresis
  • 2004
Capillary zone electrophoresis (CZE) represents a valuable method for the qualitative and quantitative assay of the most relevant polyphenol components of propolis, representing an alternative to obtain typical fingerprints of Propolis and a reliable identification of a large number of propoli polyphenolic species.