Analysis of fast dynamic processes in living cells: high-resolution and high-speed dual-color imaging combined with automated image analysis.

@article{Rustom2000AnalysisOF,
  title={Analysis of fast dynamic processes in living cells: high-resolution and high-speed dual-color imaging combined with automated image analysis.},
  author={Amin Rustom and Daniel Gerlich and R{\"u}diger Rudolf and Christian Heinemann and Roland Eils and H H Gerdes},
  journal={BioTechniques},
  year={2000},
  volume={28 4},
  pages={
          722-8, 730
        }
}
The generation of spectral mutants of the green fluorescent protein (GFP) set the stage for multiple-color imaging in living cells. However, the use of this technique has been limited by a spectral overlap of the available GFP mutants and/or by insufficient resolution in both time and space. Using a new setup for dual-color imaging, we demonstrate here the visualization of small, fast moving vesicular structures with a high time resolution. Two GFP-fusion proteins were generated: human… CONTINUE READING

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