Analysis of factor interactions with RNA polymerase II elongation complexes using a new electrophoretic mobility shift assay

@inproceedings{Cheng2008AnalysisOF,
  title={Analysis of factor interactions with RNA polymerase II elongation complexes using a new electrophoretic mobility shift assay},
  author={Bo Cheng and David H Price},
  booktitle={Nucleic acids research},
  year={2008}
}
The elongation phase of transcription by RNA polymerase II (RNAP II) is controlled by a carefully orchestrated series of interactions with both negative and positive factors. However, due to the limitations of current methods and techniques, not much is known about whether and how these proteins physically associate with the engaged polymerases. To gain insight into the detailed mechanisms involved, we established an experimental system for analyzing direct factor interactions to RNAP II… CONTINUE READING