Analysis of factor interactions with RNA polymerase II elongation complexes using a new electrophoretic mobility shift assay

@inproceedings{Cheng2008AnalysisOF,
  title={Analysis of factor interactions with RNA polymerase II elongation complexes using a new electrophoretic mobility shift assay},
  author={Bo Cheng and David H Price},
  booktitle={Nucleic acids research},
  year={2008}
}
The elongation phase of transcription by RNA polymerase II (RNAP II) is controlled by a carefully orchestrated series of interactions with both negative and positive factors. However, due to the limitations of current methods and techniques, not much is known about whether and how these proteins physically associate with the engaged polymerases. To gain insight into the detailed mechanisms involved, we established an experimental system for analyzing direct factor interactions to RNAP II… CONTINUE READING

Citations

Publications citing this paper.
SHOWING 1-10 OF 25 CITATIONS

Regulation of RNA Polymerase II Termination by Phosphorylation of Gdown1*

VIEW 4 EXCERPTS
CITES METHODS

Functional association of Gdown1 with RNA polymerase II poised on human genes.

VIEW 6 EXCERPTS
CITES METHODS

Comparative Analysis of Tat-Dependent and Tat-Deficient Natural Lentiviruses

VIEW 4 EXCERPTS
CITES BACKGROUND
HIGHLY INFLUENCED

THZ1 Reveals Roles for Cdk7 in Co-transcriptional Capping and Pausing.

VIEW 3 EXCERPTS
CITES BACKGROUND
HIGHLY INFLUENCED

References

Publications referenced by this paper.
SHOWING 1-10 OF 38 REFERENCES

A negative elongation factor for human RNA polymerase II inhibits the anti-arrest transcript-cleavage factor TFIIS.

VIEW 14 EXCERPTS

Functional coupling of capping and transcription of mRNA.

VIEW 3 EXCERPTS
HIGHLY INFLUENTIAL

Breaking barriers to transcription elongation

Mechanisms controlling CDK9 activity.