Corpus ID: 27793436

Analysis of epithelial cell surface polarity with monoclonal antibodies.

@article{Ojakian1984AnalysisOE,
  title={Analysis of epithelial cell surface polarity with monoclonal antibodies.},
  author={George K. Ojakian and Doris Herzlinger},
  journal={Federation proceedings},
  year={1984},
  volume={43 8},
  pages={
          2208-16
        }
}
  • George K. Ojakian, Doris Herzlinger
  • Published in Federation proceedings 1984
  • Chemistry, Medicine
  • The hybridoma technique of Köhler and Milstein was utilized to isolate hybrid cell lines secreting monoclonal antibodies against cell surface proteins on the Madin-Darby canine kidney (MDCK) epithelial cell line. These antibodies were employed as high-affinity ligands to study the development and maintenance of epithelial cell polarity in MDCK cells and for the identification of nephron segment-specific proteins. Using standard procedures, we were able to immunoprecipitate glycoproteins with… CONTINUE READING

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