Analysis of enzyme kinetics in individual living cells utilizing fluorescence intensity and polarization measurements.

@article{Deutsch2000AnalysisOE,
  title={Analysis of enzyme kinetics in individual living cells utilizing fluorescence intensity and polarization measurements.},
  author={Mordechai Deutsch and Morris Kaufman and Howard N. Shapiro and Naomi Zurgil},
  journal={Cytometry},
  year={2000},
  volume={39 1},
  pages={36-44}
}
BACKGROUND The Cellscan mark-S (CS-S) scanning cytometer was used for tracing enzymatic reactions in the same individual cells under various physiological conditions over periods of minutes. On-line reagent addition and changes in the experimental conditions (buffers, ions, substrates and inhibitors) were performed. METHODS Kinetic events were monitored by fluorescence intensity (FI) and fluorescence polarization (FP) measurements of fluorescein diacetate (FDA) and chloromethyl fluorescein… CONTINUE READING

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