Analysis of efficiency of two-photon versus single-photon absorption of fluorescence generation in biological objects.

Abstract

Starting from basic absorption cross-section values and breakdown limitations on possible field strengths it is shown that the total exposure required for equivalent fluorescence generation by single-photon absorption (SPA) is at least an order of magnitude lower than by two-photon absorption (TPA). The difference is such that TPA may in fact in many cases… (More)

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