Analysis of ecdysteroids by radioimmunoassay: Comparison of three different antisera

@inproceedings{Reum1979AnalysisOE,
  title={Analysis of ecdysteroids by radioimmunoassay: Comparison of three different antisera},
  author={Lutz Reum and J. Ten Doornkaat Koolman},
  year={1979}
}
Abstract Three antisera obtained with different ecdysteroid protein conjugates as antigens were studied. Antiserum DLW has the highest antibody concentration and antiserum H-21B is most sensitive for ecdysone. Antisera H-21B and ICT-1 show a high specificity for the ecdysteroid nucleus, whereas antiserum DLW is more specific for the side chain of ecdysteroids. This can be derived from cross reaction factors of ten different ecdysteroids. No antiserum is specific enough to detect ecdysone… CONTINUE READING