Analysis of ecdysteroid action in Malacosoma disstria cells: cloning selected regions of E75- and MHR3-like genes.

@article{Palli1995AnalysisOE,
  title={Analysis of ecdysteroid action in Malacosoma disstria cells: cloning selected regions of E75- and MHR3-like genes.},
  author={Subha R Palli and Sardar S Sohi and Barbara J. Cook and D. W. Lambert and Tim R Ladd and Arthur Retnakaran},
  journal={Insect biochemistry and molecular biology},
  year={1995},
  volume={25 6},
  pages={697-707}
}
IPRI-MD-66 (MD-66) cells respond to 20-hydroxyecdysone (20E, 4 x 10(-6) M) in the medium by producing cytoplasmic extensions, clumping and attaching themselves to the substrate. These morphological changes are at a maximum by 6 days post treatment. Degenerate oligonucleotides, designed on the basis of conserved amino acid sequences in the DNA and ligand binding regions of the members of the steroid hormone receptor superfamily, were used in RNA-PCR to isolate two cDNA fragments, Malacosoma… CONTINUE READING
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