Analysis of cytokine mRNA and DNA: detection and quantitation by competitive polymerase chain reaction.

@article{Gilliland1990AnalysisOC,
  title={Analysis of cytokine mRNA and DNA: detection and quantitation by competitive polymerase chain reaction.},
  author={Gary Gilliland and Steven R. Perrin and Kerry L. Blanchard and H. Franklin Bunn},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1990},
  volume={87 7},
  pages={2725-9}
}
The expression of two cytokines, granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin 3 (IL-3), has been investigated in MLA-144 cells before and after induction with phorbol 12-myristate 13-acetate. We describe an adaptation of the polymerase chain reaction (PCR) for highly accurate quantitation of mRNA or DNA from a small number of cells. Aliquots of the PCR mixture containing cDNA copies of the RNA to be assayed were added to serial dilutions of a competitor DNA fragment… CONTINUE READING
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