Analysis of clinically relevant single-nucleotide polymorphisms by use of microelectronic array technology.

@article{Santacroce2002AnalysisOC,
  title={Analysis of clinically relevant single-nucleotide polymorphisms by use of microelectronic array technology.},
  author={Rosa Santacroce and Antonia Ratti and Francesco Caroli and Barbara Foglieni and Alessandro Ferraris and Laura Cremonesi and Maurizio Margaglione and Marco Seri and Roberto Ravazzolo and Gabriella Restagno and Bruno Dallapiccola and E. Rappaport and Eleanor S. Pollak and Saul Surrey and Maurizio Ferrari and Paolo M Fortina},
  journal={Clinical chemistry},
  year={2002},
  volume={48 12},
  pages={2124-30}
}
BACKGROUND Microelectronic DNA chip devices represent an emerging technology for genotyping. We developed methods for detection of single-nucleotide polymorphisms (SNPs) in clinically relevant genes. METHODS Primer pairs, with one containing a 5'-biotin group, were used to PCR-amplify the region encompassing the SNP to be interrogated. After denaturation, the biotinylated strand was electronically targeted to discrete sites on streptavidin-coated gel pads surfaces by use of a Nanogen… CONTINUE READING