Analysis of chromatin fibers in Hela cells with electron tomography

Abstract

The presence and folding pattern of chromatin in eukaryotic cells remain elusive and controversial. In this study, we prepared ultra-thin sections of Hela cells with three different fixation and sectioning methods, i.e., chemical fixation, high pressure freezing with freeze substitution, and cryo-ultramicrotomy with SEM-FIB (focused ion beam), and analyzed… (More)
DOI: 10.1007/s41048-015-0009-9

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