Analysis of cholesterol export from endo-lysosomes by Niemann Pick C2 protein using combined fluorescence and X-ray microscopy

@article{Dupont2018AnalysisOC,
  title={Analysis of cholesterol export from endo-lysosomes by Niemann Pick C2 protein using combined fluorescence and X-ray microscopy},
  author={Alice Dupont and Frederik W. Lund and Maria Louise V. Jensen and Maria Szomek and Gitte Krogh Nielsen and Christian W Heegaard and Peter Guttmann and Stephan Werner and James G. McNally and Gerd Schneider and Sergey Kapishnikov and Daniel W{\"u}stner},
  journal={bioRxiv},
  year={2018}
}
The Niemann-Pick C2 protein (NPC2) is a sterol transfer protein in late endosomes and lysosomes (LE/LYSs). How its capacity to transport cholesterol between membranes is linked to endo-lysosomal membrane trafficking is not known. Using quantitative fluorescence imaging combined with soft X-ray tomography (SXT); we show that NPC2 mediated sterol efflux is accompanied by large changes in distribution, size and ultrastructure of endocytic organelles. We observed clearance of intra-luminal lipid… 

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TLDR
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