Analysis of cell-type-specific chromatin modifications and gene expression in Drosophila neurons that direct reproductive behavior

@article{Palmateer2020AnalysisOC,
  title={Analysis of cell-type-specific chromatin modifications and gene expression in Drosophila neurons that direct reproductive behavior},
  author={Colleen M Palmateer and Shawn C Moseley and Surjyendu Ray and Savannah G. Brovero and Michelle N. Arbeitman},
  journal={PLoS Genetics},
  year={2020},
  volume={17}
}
Examining the role of chromatin modifications and gene expression in neurons is critical for understanding how the potential for behaviors are established and maintained. We investigate this question by examining Drosophila melanogaster fru P1 neurons that underlie reproductive behaviors in both sexes. We developed a method to purify cell-type-specific chromatin (Chromatag), using a tagged histone H2B variant that is expressed using the versatile Gal4/UAS gene expression system. Here, we use… 
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A functional analysis of fru P1 circadian neurons shows they have dimorphic roles in sleep/activity and period length, and indicates that the sexes have fru P 1 neurons with common gene expression programs.
An alternative D. melanogaster 7SK snRNP
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It is proposed that two distinct 7SK genes might contribute to the formation of the 7SK snRNP complex in D. melanogaster and it is shown that, like dm7SK, this alternative 7SK interacts in vivo with the different partners of the P-TEFb complex.

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