Analysis of cat oocyte activation methods for the generation of feline disease models by nuclear transfer

  title={Analysis of cat oocyte activation methods for the generation of feline disease models by nuclear transfer},
  author={Chunmin Wang and William F. Swanson and Jason R Herrick and Kiho Lee and Zolt{\'a}n Mach{\'a}ty},
  journal={Reproductive Biology and Endocrinology : RB\&E},
  pages={148 - 148}
BackgroundSomatic cell nuclear transfer in cats offers a useful tool for the generation of valuable research models. However, low birth rates after nuclear transfer hamper exploitation of the full potential of the technology. Poor embryo development after activation of the reconstructed oocytes seems to be responsible, at least in part, for the low efficiency. The objective of this study was to characterize the response of cat oocytes to various stimuli in order to fine-tune existing and… 
Developmental competence of cat (Felis domesticus) oocytes and embryos after parthenogenetic stimulation using different methods
According to the results, parthenogenetic embryos could emerge at every step of in vitro embryo production (IVP) procedures and chemical methods for artificial activation of feline oocytes are the most promising for application to the cloning and production of parthenogenic embryos for experimental studies.
Effects of serum starvation and ionomycin activation on the development of somatic cell nuclear transfer embryos in sheep
The results of this study reveal that somatic cell synchronization by serum starvation and ionomycin treatment for the activation of oocytes can be omitted for the success of somatics cell nuclear transfer in sheep.
Design of novel oocyte activation methods: the role of zinc
Depletion of intracellular Zn2+ in oocytes with heavy metal chelators leads to successful oocyte activation in the absence of cellular Ca2+ changes, indicating that successful oocytes activation does not always depends on intrACEllularCa2+ increases.
Effects of repetitive ionomycin treatment on in vitro development of bovine somatic cell nuclear transfer embryos.
It is demonstrated that repeated ionomycin treatment is an improved activation method that can increase the developmental competence of SCNT embryos by decreasing the incidence of apoptosis.
The role of passage numbers of donor cells in the development of Arabian Oryx – Cow interspecific somatic cell nuclear transfer embryos
This study investigates the role of the passage number of the Arabian Oryx somatic cell culture when transplanted to an enucleated domestic cow oocyte and embryo development in vitro and finds the optimal passage cell number to be 10–13 Ories cell lines.
The effects of permeating cryoprotectants on intracellular free-calcium concentrations and developmental potential of in vitro-matured feline oocytes.
Evaluated the effects of exposing feline oocytes to ethylene glycol, propanediol and dimethyl sulfoxide on changes in intracellular free-calcium concentrations, the time needed for enzymatic digestion of the zona pellucida (ZP), the incidence of parthenogenetic activation and degeneration and embryonic development following in vitro fertilisation (IVF).
Cheetah interspecific SCNT followed by embryo aggregation improves in vitro development but not pluripotent gene expression.
Embryo aggregation improved the development of Ch and Dc embryos, and normalized Dc gene expression, which suggests that this strategy could improve full-term developmental efficiency of cat and feline iSCNT embryos.
Felis catus ovary as a model to study follicle biology in vitro
These data indicate, for the first time, that feline antral follicles from different stages of the natural estrous cycle can be cultured and will respond to an LH stimulus, based on an increase in steroid levels as well as C-OE after 12 or 24 h in culture.


Strategies for activating nuclear transfer oocytes.
The theories behind normal oocyte activation are reviewed as well as a number of methods of artificial oocytes activation that result in blastocyst stage embryos after oocyte maturation in vitro and artificial activation.
Mammalian oocyte activation: lessons from the sperm and implications for nuclear transfer.
Development of activation protocols that closely imitate the mechanism of activation initiated by the sperm are of special interest to improve the developmental potential of cloned embryos.
Nuclear Transfer of Synchronized African Wild Cat Somatic Cells into Enucleated Domestic Cat Oocytes1
The ability of somatic cell nuclei of the African wild cat to dedifferentiate within domestic cat (DSH) cytoplasts and to support early development after nuclear transplantation was investigated and it was revealed that the dynamics of the cell cycle varied as culture conditions were modified.
Efficient strontium-induced activation of mouse oocytes in standard culture media by chelating calcium.
Addition of EGTA to typical Ca(2+)-containing culture media can easily produce activation media that does not interfere with embryonic development and it is demonstrated that this activation method can support the birth of cloned embryos.
Activation of oocytes after nuclear transfer.
After nuclear transfer, the recipient oocyte must be stimulated to initiate development, and activation protocols were designed to target pathways downstream of the initial calcium signal to affect the activity of regulatory proteins that play central roles in maintaining developmental arrest.
Parthenogenetic activation of oocytes in c-mos-deficient mice
Results indicate that in mice Mos plays a role in the second meiotic metaphase arrest, but does not seem to be essential for the initiation of oocyte maturation, spermatogenesis or somatic cell cycle.
Parthenogenetic development and protein patterns of newly matured bovine oocytes after chemical activation
It is concluded that optimal parthenogenetic development of newly matured bovine oocytes can be obtained by calcium ionophore treatment followed by incubation in either 6‐DMAP or cycloheximide plus cytochalasin D and that the reduction of the 138‐ and 133‐kDa proteins might be necessary for the full activation of bovin oocytes.
Effect of ionomycin on oocyte activation and embryo development in mouse.
Birth of African Wildcat cloned kittens born from domestic cats.
This study evaluated the in vivo developmental competence, after transfer into domestic cat recipients, of cloned embryos produced by the fusion of African Wildcat fibroblast cell nuclei with domestic cat cytoplasts to represent the first wild carnivores to be produced by nuclear transfer.