Analysis of carbohydrate properties essential for melanogenesis in tyrosinases of cultured malignant melanoma cells by differential carbohydrate processing inhibition.

@article{Imokawa1990AnalysisOC,
  title={Analysis of carbohydrate properties essential for melanogenesis in tyrosinases of cultured malignant melanoma cells by differential carbohydrate processing inhibition.},
  author={Genji Imokawa},
  journal={The Journal of investigative dermatology},
  year={1990},
  volume={95 1},
  pages={
          39-49
        }
}
  • G. Imokawa
  • Published 1 July 1990
  • Chemistry, Medicine
  • The Journal of investigative dermatology
In order to clarify the biologic significance of carbohydrate processing in tyrosinases for melanogenesis, we have studied the effect of differential carbohydrate processing inhibitors on the recovery process of interrupted melanization which occurs after exposure of cultured B-16 melanoma cells to the inhibitor of core carbohydrate synthesis, glucosamine (Glc). Treatment of this glycosylation-dependent repigmentation process with the early-stage carbohydrate processing inhibitors… Expand
Rapid and reversible inhibition of tyrosinase activity by glucosidase inhibitors in human melanoma cells.
TLDR
Results indicate that in addition to the requirement for core glycosylation the removal of glucose residues plays a critical role in the formation of active human tyrosinase; glucosidase inhibitors appear to cause an accumulation of inactive tyrosInase and increase the degradation rate of active enzyme. Expand
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N‐linked carbohydrates in tyrosinase are required for its recognition by human MHC class II‐restricted CD4+ T cells
TLDR
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References

SHOWING 1-10 OF 33 REFERENCES
Functional analysis of tyrosinase isozymes of cultured malignant melanoma cells during the recovery period following interrupted melanogenesis induced by glycosylation inhibitors.
TLDR
Maturation of tyrosinase may occur via T1' and T1'' as precursors of T3, or possibly T1 through the addition of N-glycosydically linked oligosaccharide moieties which can be interrupted by glucosamine and tunicamycin. Expand
Loss of melanogenic properties in tyrosinases induced by glucosylation inhibitors within malignant melanoma cells.
Glycosylation inhibitors, glucosamine or tunicamycin, have been found to be specific inhibitory modulators for melanogenesis, which is accentuated generally in malignant melanoma cells. Exposure toExpand
Importance of glycoproteins in the initiation of melanogenesis: an electron microscopic study of B-16 melanoma cells after release from inhibition of glycosylation.
TLDR
2 possible mechanisms for the involvement of glycosylation in melanogenesis are suggested: first, the translocation of tyrosinases may be regulated by the presence of specific carbohydrate moieties; second, melanosomal matrix proteins contain carbohydrates which may contribute to the tyrosInase-accepting function or in vivo melanizing function of tyosinases after forming particle-bound T3 tyros inase. Expand
Purification of hamster melanoma tyrosinases and structural studies of their asparagine-linked sugar chains.
TLDR
In cultured melanotic melanoma, a marked decrease of pigmentation has been found to be induced by the addition of tunicamycin, and it appears that this impaired pigmentation arises from the loss of asparagine-linked sugar chains serving as a signal for transport of tyrosinase from GERL to premelanosomes. Expand
Analysis of initial melanogenesis including tyrosinase transfer and melanosome differentiation through interrupted melanization by glutathione.
  • G. Imokawa
  • Chemistry, Medicine
  • The Journal of investigative dermatology
  • 1989
TLDR
It is suggested that glutathione provides a new situation of interrupted melanogenesis in which melanization cannot proceed despite complete formation of melanosome matrix structure and a lack of inhibition of cellular metabolisms including protein glycosylation. Expand
Analysis of tyrosinases as asparagin-linked oligosaccharides by concanavalin A lectin chromatography: appearance of new segment of tyrosinases in melanoma cells following interrupted melanogenesis induced by glycosylation inhibitors.
TLDR
Findings suggest that altered structures of carbohydrate moiety in tyrosinase molecules play a role in the induction of loss of membrane-binding capacity of tyosinases, resulting in the loss of melanization in pigment cells. Expand
Selective aberration and pigment loss in melanosomes of malignant melanoma cells in vitro by glycosylation inhibitors: premelanosomes as glycoprotein.
TLDR
It is concluded that glycoprotein synthesis is integral to the formation of normal melanosomes and to their specific melanizing function, which could be impaired by inhibition of the synthesis of asparagin-linked mannose-containing sugar chains. Expand
Isolation and biochemical characterization of tyrosinase‐rich GERL and coated vesicle in melanin synthesizing cells
TLDR
The site of condensation and activation of tyrosinase prior to melanosome melanogenesis has been investigated using subcellular fractionation and enzymic analysis of so‐called Golgi apparatus of melanoma cells and suggests that the GERL‐coated vesicle unit is a specialized portion of smooth endoplasmic reticulum having the function of activating and condensing the tyros inase synthesized by ribosomes. Expand
Inhibition of processing of plant N-linked oligosaccharides by castanospermine.
TLDR
In plant cells, castanospermine also prevents the removal of the outermost glucose residue as well as inhibiting glucosidase I and leads to altered glycoproteins with Glc3Man7GlcNAc2 structures. Expand
Effect of swainsonine on the processing of the asparagine-linked carbohydrate chains of alpha 1-antitrypsin in rat hepatocytes. Evidence for the formation of hybrid oligosaccharides.
The biosynthesis of the proteinase inhibitor alpha 1-antitrypsin has been studied in rat hepatocyte primary cultures. Newly synthesized alpha 1-antitrypsin was found in hepatocytes as a glycoproteinExpand
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