Analysis of binding specificity of disulfide bonded dimeric lambda-Cro V55C protein with generic hexamer oligonucleotide microchip.

@article{Chechetkin2003AnalysisOB,
  title={Analysis of binding specificity of disulfide bonded dimeric lambda-Cro V55C protein with generic hexamer oligonucleotide microchip.},
  author={Vladimir R. Chechetkin and Dmitry Prokopenko and Olga A. Zasedateleva and G I Gitelson and E S Lomakin and Mikhail A. Livshits and Lucy Malinina and A. Yu. Turygin and A. S. Krylov and A. D. Mirzabekov},
  journal={Journal of biomolecular structure & dynamics},
  year={2003},
  volume={21 3},
  pages={425-33}
}
Binding specificity of mutant V55C disulfide bonded dimeric lambda-Cro protein (CroVC) to double-stranded DNA (dsDNA) was studied using generic hexamer oligonucleotide microchip. The curves of dissociation of hybridized DNA in the presence and absence of CroVC were converted into the effective discriminant constants to assess the relevant thermodynamic equilibrium binding constants for dsDNA-protein complexes. Then, tiling of longer oligonucleotides with shorter oligomers was used to search for… CONTINUE READING