Analysis of baculovirus aggregates using flow cytometry.

  title={Analysis of baculovirus aggregates using flow cytometry.},
  author={Hasnaa Jorio and Rosa Tran and Jamal Meghrous and Lucie Bourget and Amine A Kamen},
  journal={Journal of virological methods},
  volume={134 1-2},
Aggregation of viral particles represents a significant problem for baculoviral stock processing and storage. Aggregation may also affect the results of viral particle counting. A method using flow cytometry was previously developed in our lab to measure the concentration of baculovirus particles produced in insect cell cultures. In the present study, the use of the flow cytometry method was extended to the detection of baculovirus aggregates. Flow cytometry analysis of freshly prepared… CONTINUE READING

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