Analysis of bacteriophage P 1 immunity by using XP 1 recombinants constructed in vitro ( P 1 repressor genes / PI antirepressor gene / PI dnaB analog gene / DNA cloning )

Abstract

We describe the dissection and reconstruction of a complex control circuit, the P1 immunity system, by a method that involves inserting EcoRI-generated fragments of P1 DNA into X vectors that can then be sequentially inserted into a bacterial cell. Using these techniques we have isolated X-P1 hybrid phages that express the products of P1 genes cl, c4, ant… (More)

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