Analysis of an activator:coactivator complex reveals an essential role for secondary structure in transcriptional activation.

@article{Parker1998AnalysisOA,
  title={Analysis of an activator:coactivator complex reveals an essential role for secondary structure in transcriptional activation.},
  author={Denise Parker and Ulupi S. Jhala and Ishwar Radhakrishnan and Michael B. Yaffe and Christine M. Reyes and Andrew I. Shulman and Lewis C. Cantley and Peter E. Wright and Marc R. Montminy},
  journal={Molecular cell},
  year={1998},
  volume={2 3},
  pages={353-9}
}
Ser-133 phosphorylation of CREB within the kinase-inducible domain (KID) promotes target gene activation via complex formation with the KIX domain of the coactivator CBP. Concurrent phosphorylation of CREB at Ser-142 inhibits transcriptional induction via an unknown mechanism. Unstructured in the free state, KID folds into a helical structure upon binding to KIX. Using site-directed mutagenesis based on the NMR structure of the KID:KIX complex, we have examined the mechanisms by which Ser-133… CONTINUE READING