Analysis of amino acids in biological fluids by pentafluorobenzyl chloroformate derivatization and detection by electron capture negative ionization mass spectrometry.

@article{Simpson1996AnalysisOA,
  title={Analysis of amino acids in biological fluids by pentafluorobenzyl chloroformate derivatization and detection by electron capture negative ionization mass spectrometry.},
  author={J. T. Simpson and D. S. Torok and J. Girard and S. Markey},
  journal={Analytical biochemistry},
  year={1996},
  volume={233 1},
  pages={
          58-66
        }
}
Pentafluorobenzyl chloroformate (PFBCF) has been utilized as a derivatization reagent for amino acids (AAs) in biological fluids with susequent detection by electron capture negative ionization mass spectrometry (ECNI/MS). AAs were derivatized in one step in aqueous solution, plasma, and whole blood at room temperature. To demonstrate quantitative analysis, phenylalanine concentrations were determined in human plasma. AAs were derivatized in one step using PFBCF and a mixture of water, ethanol… Expand
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