Analysis of amino acids in biological fluids by pentafluorobenzyl chloroformate derivatization and detection by electron capture negative ionization mass spectrometry.

  title={Analysis of amino acids in biological fluids by pentafluorobenzyl chloroformate derivatization and detection by electron capture negative ionization mass spectrometry.},
  author={J. T. Simpson and D. S. Torok and J. Girard and S. Markey},
  journal={Analytical biochemistry},
  volume={233 1},
Pentafluorobenzyl chloroformate (PFBCF) has been utilized as a derivatization reagent for amino acids (AAs) in biological fluids with susequent detection by electron capture negative ionization mass spectrometry (ECNI/MS). AAs were derivatized in one step in aqueous solution, plasma, and whole blood at room temperature. To demonstrate quantitative analysis, phenylalanine concentrations were determined in human plasma. AAs were derivatized in one step using PFBCF and a mixture of water, ethanol… Expand
Fluoroalkyl chloroformates in treating amino acids for gas chromatographic analysis.
The novel fluoroalkyl chloroformates with three and four carbon atoms were investigated for the immediate conversion of amino acids into hydrophobic derivatives in water-containing media, enabling to treat amino acids in aqueous environment and to analyze them in <15 min. Expand
1.1.1. - Quantitation of Amino Acids as Chloroformates – A Return to Gas Chromatography
Summary Alkyl chloroformates (RCF) in aqueous alcohol-pyridine (PYR) media enable amino acids (AAs) to be converted into derivatives amenable to gas chromatography (GC) analysis in seconds. There isExpand
Chloroformates in gas chromatography as general purpose derivatizing agents.
  • P. Hušek
  • Medicine, Chemistry
  • Journal of chromatography. B, Biomedical sciences and applications
  • 1998
Reaction conditions were optimized for various classes of carboxylic acids and a uniquely rapid derivatization of amino acids in aqueous ethanol was shown to be possible and most of the analytes, e.g. acidic metabolites in physiological fluids, could be treated directly in the aqueously matrix. Expand
Derivatization in Mass Spectrometry—6. Formation of Mixed Derivatives of Polyfunctional Compounds
Application of mixed derivatization to qualitative and quantitative analysis of various multifunctional compounds mainly in biological fluids and other matrices by gas chromatography/mass spectrometry in electron ionization, chemical ions, negative-ion chemical ionization and selected ion monitoring modes is considered. Expand
Chemical derivatization of amino acids for in situ analysis of Martian samples by gas chromatography.
Three different methods of derivatization are tested in order to select and optimize one for the in situ analysis of amino acids in Martian samples, which has several advantages such as one-step reaction and short time analysis. Expand
Improved Procedure for n-Hexyl Chloroformate-Mediated Derivatization of Highly Hydrophilic Substances Directly in Water: Hydroxyaminic Compounds
Abstract A fast derivatization procedure, carried out directly on aqueous solutions of highly hydrophilic substances, has been modified and improved in order to determine amino alcohols,Expand
Determination of airborne methamphetamine via capillary microextraction of volatiles (CMV) with on-sorbent derivatisation using o-pentafluorobenzyl chloroformate
Abstract Capillary microextraction of volatiles (CMV), a high surface area sampling technique, was combined with on-sorbent derivatisation to develop a sensitive alternative to dynamic solid phaseExpand
Derivatization in Mass Spectrometry—2. Acylation
The present review is devoted to acylation as a widely employed derivatization procedure for protection of OH (alcohols, polyols, phenols, enols), SH (thiols) and NH (amines, amides) groups in orderExpand
Separation of amino acids by ion mobility spectrometry.
The mobilities of the 20 common amino acids were determined by electrospray ionization ion mobility spectrometry. It was found that each amino acid had a different drift time and hence a differentExpand
Derivatization in Mass Spectrometry—1. Silylation
Advances in silylation as a powerful tool used for increasing the volatility, thermal and thermo-catalytic stability, and chromatographic mobility of polar and unstable organic compounds are described. Expand