• Chemistry, Medicine
  • Published in
    Rapid communications in mass…
    2001
  • DOI:10.1002/rcm.501

Analysis of amino acids as formamidene butyl esters by electrospray ionization tandem mass spectrometry.

@article{Johnson2001AnalysisOA,
  title={Analysis of amino acids as formamidene butyl esters by electrospray ionization tandem mass spectrometry.},
  author={David W Johnson},
  journal={Rapid communications in mass spectrometry : RCM},
  year={2001},
  volume={15 22},
  pages={
          2198-205
        }
}
Amino acid formamidene butyl esters are optimally prepared by heating amino acids with dimethylformamide dimethylacetal (DMF-DMA) for 2 minutes at 65 degrees C and then with n-butanol/hydrogen chloride for 15 minutes at 65 degrees C. The formamidene butyl esters of simple alpha-amino acids and beta-amino acids afford between 1.1 and 20 times the signal intensity of the corresponding butyl esters during electrospray ionization tandem mass spectral analysis. Complex alpha-amino acids, such as… CONTINUE READING

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