Analysis of alphavirus polypeptides by two dimensional polyacrylamide gel electrophoresis

Abstract

Semliki Forest, Sindbis and Chikungunya viruses were grown and radio-labeled with [3H]-amino acids in Vero cells. Analysis of virus infected cell lysates by two dimensional polyacrylamide gel electrophoresis resulted in detection of polypeptides of molecular, weights corresponding to those of E1, P62, ns60, ns70/72 for Semliki Forest virus, the C, E1, 6K, 14K, PE2, P97, ns60, ns82 for Sindbis virus and E1. P62, P97, ns70/72 for Chikungunya virus. Charge and molecular weight heterogeneity in the precursor polypeptide P62 of Semliki Forest virus was detected. Structural poly-peptides e.g. E1 and E2 of Semliki Forest virus and C, E1, E2 of Sindbis virus and E1 of Chikungunya virus were detected when purified radiolabeled virus preparations were analyzed by two dimensional polyacrylamide gel-electrophoresis. Membrane glycoprotein E1 and E2 of Semliki Forest and E1 of Sindbis and Chikungunya viruses exhibited charge heterogeneity. In contrast to the marked difference in isoelectric points of E1 and E2 of Sindbis virus; E1 and E2 of Semliki Forest virus had almost identical isoelectric points.

DOI: 10.1007/BF02716601

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@article{Deshmane2007AnalysisOA, title={Analysis of alphavirus polypeptides by two dimensional polyacrylamide gel electrophoresis}, author={Satish Deshmane and Kalyan Banerjee}, journal={Journal of Biosciences}, year={2007}, volume={5}, pages={189-201} }