Analysis of a specific oxygenation reaction of soybean lipoxygenase-1 with fatty acids esterified in phospholipids.

@article{Brash1987AnalysisOA,
  title={Analysis of a specific oxygenation reaction of soybean lipoxygenase-1 with fatty acids esterified in phospholipids.},
  author={Alan R. Brash and Christiana D. Ingram and Thomas M. Harris},
  journal={Biochemistry},
  year={1987},
  volume={26 17},
  pages={5465-71}
}
Soybean lipoxygenase was reacted with phosphatidylcholine (at pH 9, with 10 mM deoxycholate), and the oxygenation products were analyzed by high-pressure liquid chromatography, UV, gas chromatography-mass spectrometry (GC-MS), and NMR. The structures of the intact glycerolipid products were established by GC-MS of diglycerides recovered by phospholipase C… CONTINUE READING