Analysis of a shortened form of human carbonic anhydrase VII expressed in vitro compared to the full-length enzyme.

@article{Bootorabi2010AnalysisOA,
  title={Analysis of a shortened form of human carbonic anhydrase VII expressed in vitro compared to the full-length enzyme.},
  author={Fatemeh Bootorabi and Janne J{\"a}nis and Elona Smith and Abdul Waheed and Sampo Kukkurainen and Vesa P. Hyt{\"o}nen and Jarkko Valjakka and Claudiu T. Supuran and Daniela Vullo and William S. Sly and Seppo Parkkila},
  journal={Biochimie},
  year={2010},
  volume={92 8},
  pages={1072-80}
}
Carbonic anhydrase (CA) enzymes are expressed in all organs of the mammalian body where they participate in important physiological functions. CA VII is a cytosolic isozyme which may be expressed as two forms according to the recent GenBank data. We designed a present study to express and characterize the human CA VII forms: full-length CA VII and short form (predicted to lack 56 residues from the N-terminus). Reverse transcriptase PCR analysis revealed mRNAs for both CA VII forms in the human… CONTINUE READING