Analysis of a nucleoprotein complex: the synaptosome of gamma delta resolvase.

@article{Grindley1993AnalysisOA,
  title={Analysis of a nucleoprotein complex: the synaptosome of gamma delta resolvase.},
  author={Nigel D. F. Grindley},
  journal={Science},
  year={1993},
  volume={262 5134},
  pages={
          738-40
        }
}
The gamma delta resolvase protein is one of a large family of transposon-encoded site-specific recombinases. It performs recombination in a DNA-protein complex that contains 12 resolvase protomers and two copies of the 120-base pair DNA substrate, res (each with three binding sites for a resolvase dimer). A derivative of resolvase with altered DNA binding specificity was used to show that the role of resolvase at site I, which contains the crossover point, differs from its role at the other two… CONTINUE READING