Analysis of a high frequency transformation system for Ophiostoma ulmi, the causal agent of Dutch elm disease

@article{Royer2004AnalysisOA,
  title={Analysis of a high frequency transformation system for Ophiostoma ulmi, the causal agent of Dutch elm disease},
  author={J. Royer and K. Dewar and M. Hubbes and P. A. Horgen},
  journal={Molecular and General Genetics MGG},
  year={2004},
  volume={225},
  pages={168-176}
}
SummaryA transformation system for Ophiostoma ulmi (Buism.) Nannf. was developed and analyzed. Protoplasts were generated from actively budding yeastlike cells by digestion with NovoZym 234 in MgSO4 after pretreatment with 2-mercaptoethanol. Protoplast regeneration was most efficient when 0.6 M sucrose was used as the osmoticum. Several plasmids containing fusions between fungal promoters and a bacterial gene for hygromycin phosphotransferase successfully transformed O. ulmi to hygromycin… Expand
Use of insertional mutagenesis to tag putative parasitic fitness genes in the Dutch elm disease fungus Ophiostoma novo-ulmi subsp. novo-ulmi.
Heterologous transformation of Zalerion arboricola
Stress-induced mobility of OPHIO1 and OPHIO2, DNA transposons of the Dutch elm disease fungi.
Transformation of Rhizoctonia solani
Electrophoretic karyotypes of the elm tree pathogen Ophiostoma ulmi (sensu lato)
Inheritance of chromosome-length polymorphisms in Ophiostoma ulmi (sensu lato)
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