Analysis of a high frequency transformation system for Ophiostoma ulmi, the causal agent of Dutch elm disease

@article{Royer1991AnalysisOA,
  title={Analysis of a high frequency transformation system for Ophiostoma ulmi, the causal agent of Dutch elm disease},
  author={John C. Royer and Ken Dewar and Martin Hubbes and Paul A. Horgen},
  journal={Molecular and General Genetics MGG},
  year={1991},
  volume={225},
  pages={168-176}
}
SummaryA transformation system for Ophiostoma ulmi (Buism.) Nannf. was developed and analyzed. Protoplasts were generated from actively budding yeastlike cells by digestion with NovoZym 234 in MgSO4 after pretreatment with 2-mercaptoethanol. Protoplast regeneration was most efficient when 0.6 M sucrose was used as the osmoticum. Several plasmids containing fusions between fungal promoters and a bacterial gene for hygromycin phosphotransferase successfully transformed O. ulmi to hygromycin… CONTINUE READING
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Electrophoretic karyotypes of the elm tree pathogen Ophiostoma ulmi (sensu lato)

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  • 1993
VIEW 3 EXCERPTS
CITES METHODS
HIGHLY INFLUENCED

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  • Journal of Wood Science
  • 2003
VIEW 2 EXCERPTS
CITES BACKGROUND & RESULTS

References

Publications referenced by this paper.
SHOWING 1-10 OF 52 REFERENCES

Gene transfer system for the phytopathogenic fungus Ustilago maydis.

  • Proceedings of the National Academy of Sciences of the United States of America
  • 1988
VIEW 3 EXCERPTS
HIGHLY INFLUENTIAL

Transformation of the fungal pathogen Cryphonectria parasitica with a variety of heterologous plasmids

RN Cooley, RK Shaw, Franklin FCH, CE Caten
  • Curr Genet
  • 1990

Current protocols in molecular biology unit 2.5B, pulsed-field gel electrophoresis

FM Ausubel, R Brent, +4 authors K Struhl
  • 1989
VIEW 1 EXCERPT

Current protocols in molecular biology unit 2.5B, pulsed-field gel electrophoresis

FM Ausubel, R Brent, +4 authors K Struhl
  • 1989
VIEW 1 EXCERPT

New varieties of elm raise hope of rebirth for devastated tree

S Asina, M Hubbes
  • 1989

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