Analysis of a commercially improved Penicillium chrysogenum strain series: involvement of recombinogenic regions in amplification and deletion of the penicillin biosynthesis gene cluster

@article{Newbert1997AnalysisOA,
  title={Analysis of a commercially improved Penicillium chrysogenum strain series: involvement of recombinogenic regions in amplification and deletion of the penicillin biosynthesis gene cluster},
  author={Roger W. Newbert and Barry Barton and Philip Greaves and Joyce Harper and Gwen Turner},
  journal={Journal of Industrial Microbiology and Biotechnology},
  year={1997},
  volume={19},
  pages={18-27}
}
Several commercially improved strains of Penicillium chrysogenum have been shown to carry amplifications of the entire penicillin biosynthesis gene cluster. Analysis previously carried out using the strain BW 1890 has here been extended to the characterisation of other members of the SmithKline Beecham strain improvement series. We have determined the length of the amplicon to be 57.4 kb and shown a general increase in copy number and penicillin titre through the series. Sequence analyses of… CONTINUE READING

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