Analysis of a bacterial hygromycin B resistance gene by transcriptional and translational fusions and by DNA sequencing.

@article{Kaster1983AnalysisOA,
  title={Analysis of a bacterial hygromycin B resistance gene by transcriptional and translational fusions and by DNA sequencing.},
  author={Kevin R. Kaster and Stanley G. Burgett and R. Nagaraja Rao and Thomas D. Ingolia},
  journal={Nucleic acids research},
  year={1983},
  volume={11 19},
  pages={6895-911}
}
We have characterized hygromycin B and apramycin resistance genes from an E. coli plasmid. We have localized the coding and control regions of these genes by deletion of DNA fragments from plasmids containing the genes. It was found that polypeptides with apparent molecular weights of 33,000 and 31,500 daltons are encoded by the apramycin resistance gene and polypeptides with apparent molecular weights of 42,500 and 41,500 daltons are encoded by the hygromycin B resistance gene. DNA sequence… CONTINUE READING
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