Analysis of UVB‐modulated Gene Expression in Human Keratinocytes by mRNA Differential Display Polymerase Chain Reaction

@article{Abts1997AnalysisOU,
  title={Analysis of UVB‐modulated Gene Expression in Human Keratinocytes by mRNA Differential Display Polymerase Chain Reaction},
  author={Harry F. Abts and Kai Breuhahn and G{\"u}nter Michel and Karl Kohrer and Peter J. Esser and Thomas Ruzicka},
  journal={Photochemistry and Photobiology},
  year={1997},
  volume={66}
}
Abstract— EUltraviolet (UV) light is the most important environmental insult to skin. Even a single exposure to UVB radiation can result in inflammation and may also lead to DNA damage and apoptosis in the acute response of the cutaneous tissue. To elucidate the complex alterations of gene expression in human keratinocytes underlying these UV responses we took advantage of differential display polymerase chain reaction (DD‐PCR) technology's ability to detect qualitative and quantitative changes… Expand
Modulation of gene expression by solar ultraviolet radiation
TLDR
In vivo studies in human skin have been mainly directed towards understanding photoaging caused by chronic exposure to sunlight and it is now well-established that induction of matrix metalloproteinases by keratinocytes and fibroblasts leads to degradation of the extracellular matrix. Expand
Analysis of Gene Regulation in Rabbit Corneal Epithelial Cells Induced by Ultraviolet Radiation
TLDR
Data showed that FDDRT-PCR is a useful technique to elucidate UV-regulated gene expressions and could lead to a better understanding of cataract formation via DNA damage and mechanisms of prevention. Expand
A novel immediate early response gene, IEX-1, is induced by ultraviolet radiation in human keratinocytes.
TLDR
It is shown that in a dose-dependent manner, UVR induces the expression of messenger RNA of a novel immediate early response gene, IEX-1, in human keratinocytes, which may play a role in keratinocyte proliferation especially following UVR. Expand
Search for UV-responsive genes in human cells by differential mRNA display: involvement of human ras-related GTP-binding protein, Rheb, in UV susceptibility.
  • K. Kita, Y. Wu, +6 authors N. Suzuki
  • Biology, Medicine
  • Biochemical and biophysical research communications
  • 2000
TLDR
The results of antisense experiments together with the up-regulation in UV-irradiated RSa cells, suggest that Rheb is involved in the UV sensitization of both cells to UV cell-killing. Expand
UVB and γ‐Radiation Induce the Expression of mRNAs Encoding the Ribosomal Subunit L13A in Rat Keratinocytes
TLDR
Observations suggest that increased expression of ribosomal subunit genes may be a molecular component of the keratinocyte response to UVB in particular and not part of a nonspecific response to DNA damage. Expand
Transcriptional activation of endogenous retroviral sequences in human epidermal keratinocytes by UVB irradiation.
TLDR
The activation of human endogenous retroviral sequence transcription by ultraviolet B may contribute to the pathogenesis of lupus erythematosus, where inappropriate antigenic presentation of ultraviolet B-induced viral and cellular proteins could stimulate autoantibody production. Expand
Solar ultraviolet radiation as a trigger of cell signal transduction.
TLDR
The present review summarizes some of the mechanisms by which ultraviolet light alters signaling pathways as well as the genes important in the beneficial and toxic effects of ultraviolet light. Expand
HAX-1, identified by differential display reverse transcription polymerase chain reaction, is overexpressed in lesional psoriasis.
TLDR
In psoriasis, the epidermal differentiation could be disturbed due to the increased expression of HAX-1 and hence a prolonged resistance to terminal differentiation, and antiapoptotic mechanisms are an emerging concept for the understanding of the pathogenesis of this disease possibly leading to clinical applications. Expand
Proteome analysis of ultraviolet‐B‐induced protein expression in vitro human dermal fibroblasts
TLDR
This study investigated UVB‐induced alterations in protein expression in human dermal fibroblasts through the induction of reactive oxygen species, DNA damage, activation of signaling pathways, and regulation of gene expression. Expand
Molecular basis of basal cell carcinoma: Analysis of differential gene expression by differential display PCR and expression array
TLDR
Differential display RT‐PCR and differential hybridization of cDNA arrays allow the detection of differentially expressed genes possibly involved in the carcinogenesis of BCC. Expand
...
1
2
3
...

References

SHOWING 1-10 OF 36 REFERENCES
UVB radiation-activated genes induced by transcriptional and posttranscriptional mechanisms in rat keratinocytes.
TLDR
The identification of a group of UVB-inducible keratinocyte genes should prove useful for the characterization of the genomic response to UVB radiation and the analysis of the molecular mechanisms underlying the UVB regulation of gene expression. Expand
IL-8 gene expression and production in human keratinocytes and their modulation by UVB.
TLDR
A possible role for IL-8 in UVB-induced skin inflammation and diseases is suggested as it is known to be an inflammatory cell chemotactic factor. Expand
Effects of UV, 4-NQO and TPA on gene expression in cultured human epidermal keratinocytes.
TLDR
Results indicate that in keratinocytes UV irradiation leads to a relative increase in the level of some mRNAs, corresponding to the majority of the isolated cDNA clones. Expand
Ultraviolet B radiation up-regulates the expression of IL-15 in human skin.
TLDR
A new mechanism by which UVB can promote Ag-independent T cell responses via elaboration of IL-15 is suggested, in addition to expanding the known influence of UVB radiation on the capacity of keratinocytes and dermal fibroblasts to express immunomodulatory cytokines. Expand
Molecular basis of sun-induced premature skin ageing and retinoid antagonism
TLDR
It is proposed that elevated metalloprotein-ases, resulting from activation of AP-1 and NF-KB by low-dose solar irradiation, degrade collagen and elastin in skin, which would result in solar scars, which through accumulation from a lifetime of repeated low- dose sunlight exposure could cause premature skin ageing (photoageing). Expand
Rapid and preferential activation of the c-jun gene during the mammalian UV response.
TLDR
It is found that expression of both c-jun and c-fos, which encode proteins that participate in formation of the AP-1 complex, is rapidly induced by two different DNA-damaging agents: UV and H2O2. Expand
Expression of ribosomal phosphoprotein PO is induced by antitumor agents and increased in Mer- human tumor cell lines.
TLDR
It is shown by Northern analysis that PO is induced by L-phenylalanine mustard, 4-hydroperoxycyclophosphamide and mechlorethamine, which are DNA-damaging agents commonly used as chemotherapeutic antitumor agents. Expand
Isolation of DAP3, a Novel Mediator of Interferon-γ-induced Cell Death (*)
TLDR
The data presented suggest that DAP3 is a positive mediator of cell death induced by interferon-γ, and this gene is a functional selection approach of gene cloning that is based on transfection with an antisense cDNA expression library. Expand
Ultraviolet Radiation Effects on Human Keratinocyte ICAM-1 Expression: UV-Induced Inhibition of Cytokine-Induced ICAM-1 mRNA Expression Is Transient, Differentially Restored for IFNγ Versus TNFα, and Followed by ICAM-1 Induction Via a TNFα-Like Pathway
TLDR
UV irradiation is identified as a potent modulator of cytokine regulated ICAM-1 gene transcription with the capacity to induce both inhibitory as well as enhancing effects. Expand
UV activation of receptor tyrosine kinase activity.
TLDR
It is demonstrated that UV rapidly activates both the EGF receptor and insulin receptor, as shown by tyrosine phosphorylation of these receptors, and a critical role for receptor-mediated events in regulating the response mammalian cells to UV exposure is demonstrated. Expand
...
1
2
3
4
...