Corpus ID: 87422166

Analysis of Squalene Synthase Expression During the Development of Ganoderma lucidum

@article{Zhao2004AnalysisOS,
  title={Analysis of Squalene Synthase Expression During the Development of Ganoderma lucidum},
  author={M. W. Zhao and Jiayu Zhong and Wanqi Liang and N. Wang and M. J. Chen and Dabing Zhang and Y. J. Pan and Shung C. Jong},
  journal={Journal of Microbiology and Biotechnology},
  year={2004},
  volume={14},
  pages={116-120}
}
The medicinal properties of Ganoderma lucidum have been recognized in China for many centuries. Active pharmaceutical components include triterpenes. To elucidate the molecular regulation of triterpene biosynthesis in this mushroom, a 57-base pair DNA fragment encoding the fourth conserved domain SQ-4 (SMGLFLQKTNIIRDYNEDL) of squalene synthase was synthesized and cloned into the expression vector pET-32a(+). The recombinant fusion protein induced by IPTG (isopropyl-β-D-thiogalactopyranoside… Expand

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