Analysis of Sodium Transport in the Amphibian Oocyte by Extractive and Radioautographic Techniques

@article{Horowitz1970AnalysisOS,
  title={Analysis of Sodium Transport in the Amphibian Oocyte by Extractive and Radioautographic Techniques},
  author={Samuel B. Horowitz and I. Robert Fenichel},
  journal={The Journal of Cell Biology},
  year={1970},
  volume={47},
  pages={120 - 131}
}
The transport of Na(+) in mature Eurycea oocytes was studied by quantitative radioautography of (22)Na(+) using techniques suitable for localization of diffusible solutes, together with conventional extractive techniques. Intracellular Na(+) consisted of three kinetic fractions: a cytoplasmic fast fraction of about 8.5 microeq/ml H(2)O; a cytoplasmic slow fraction of about 58.7 microeq/ml H(2)O; and a nuclear fast fraction of about 11.1 microeq/ml H(2)O. A nuclear slow fraction, if it exists… CONTINUE READING