Analysis of RNA-mediated gene silencing using a new vector (pKNOCKOUT) and an in plantaAgrobacterium transient expression system

@article{Cazzonelli2007AnalysisOR,
  title={Analysis of RNA-mediated gene silencing using a new vector (pKNOCKOUT) and an in plantaAgrobacterium transient expression system},
  author={Christopher Ian Cazzonelli and Jeff P Velten},
  journal={Plant Molecular Biology Reporter},
  year={2007},
  volume={22},
  pages={347-359}
}
A hairpin RNA (hpRNA) vector, pKNOCKOUT (pKO) has been constructed to facilitate the analysis of posttranscriptional gene silencing (PTGS) in anAgrobacterium-mediated transient expression system developed for tobacco. The pKO binary vector was tested by cloning a firefly luciferase (Photinus pyralis) gene segment in sense (sFLUC), antisense (aFLUC), and inverted repeat (ihpFLUC) orientations. The inverted repeats of the target gene are separated by the castor bean catalase intron (CbCi) and… CONTINUE READING

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