Analysis of RNA cleavage by MALDI-TOF mass spectrometry

  title={Analysis of RNA cleavage by MALDI-TOF mass spectrometry},
  author={Jeff C. Joyner and Kevin D. Keuper and John A Cowan},
  booktitle={Nucleic acids research},
A method of analysis is presented that utilizes matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) to monitor the kinetics and products of RNA cleavage, by use of a program designed to mass-match observed MS peaks with predicted RNA cleavage products. The method is illustrated through application to the study of targeted oxidation of RNA stem loops from HIV-1 Rev Response Element mRNA (RRE RNA) and ribosomal 16S A-site RNA (16S RNA) by metallonucleases… CONTINUE READING