Analysis of RNA chain elongation and termination by Saccharomyces cerevisiae RNA polymerase III.

@article{Matsuzaki1994AnalysisOR,
  title={Analysis of RNA chain elongation and termination by Saccharomyces cerevisiae RNA polymerase III.},
  author={Hiroaki Matsuzaki and George A. Kassavetis and E. Peter Geiduschek},
  journal={Journal of molecular biology},
  year={1994},
  volume={235 4},
  pages={1173-92}
}
When Saccharomyces cerevisiae RNA polymerase (Pol) III transcribes the S. cerevisiae SUP4 tRNA(Tyr) gene, it is obliged to navigate past a large, multi-subunit DNA-bound complex of proteins. We have analyzed individual steps of RNA chain elongation on this gene. Slow steps of transcriptional initiation were by-passed by forming 5'-end-labeled, arrested and precisely positioned transcription complexes. Synchronous resumption of chain elongation by these complexes allowed a single round of RNA… CONTINUE READING
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