Analysis of Polar Compounds Using 100% Aqueous Mobile Phases with Agilent ZORBAX Eclipse Plus Phenyl-Hexyl and Other ZORBAX Phenyl Columns

Abstract

The analysis of polar compounds, such as acids and bases, typically requires ion pairing reagents or Hydrophobic Interaction Chromatography (HILIC) columns in HPLC. In this work, the utility of phenyl columns is demonstrated using 100% aqueous mobile phase, without the inconvenience of phase collapse. Selectivity of different Agilent ZORBAX phenyl phases… (More)

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