Analysis of Pirlimycin Residues in Beef Muscle, Milk, and Honey by a Biotin-Streptavidin-Amplified Enzyme-Linked Immunosorbent Assay.

@article{Jiang2016AnalysisOP,
  title={Analysis of Pirlimycin Residues in Beef Muscle, Milk, and Honey by a Biotin-Streptavidin-Amplified Enzyme-Linked Immunosorbent Assay.},
  author={Wenxiao Jiang and Ross C. Beier and Pengjie Luo and Peng Zhai and Nan Wu and Guimiao Lin and Xiao-mei Wang and Gaixia Xu},
  journal={Journal of agricultural and food chemistry},
  year={2016},
  volume={64 1},
  pages={
          364-70
        }
}
Food contamination by veterinary drug residues is a worldwide public health concern and requires continuous monitoring. In this study, we developed a biotin-streptavidin-amplified ELISA (BA-ELISA) using a produced monoclonal antibody for detecting pirlimycin residues in beef muscle, milk, and honey. The IC50 value of the BA-ELISA was 1.6 ng/mL for pirlimycin in buffer, and the sensitivity was improved 3 times compared to traditional ELISAs. The optimized BA-ELISA can be used to quantitate trace… CONTINUE READING
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