Analysis of OmpC-beta-lactamase hybrid proteins: OmpC appears not to contain discrete localization signals.

@article{Saarilahti1990AnalysisOO,
  title={Analysis of OmpC-beta-lactamase hybrid proteins: OmpC appears not to contain discrete localization signals.},
  author={Hannu T. Saarilahti and E Tapio Palva},
  journal={FEMS microbiology letters},
  year={1990},
  volume={57 1-2},
  pages={49-54}
}
Fusions to the beta-lactamase (bla) gene were employed to analyze the presence of localization information in the mature part of OmpC, a major pore-forming outer membrane protein in Escherichia coli K-12. Six translational ompC-bla gene fusions were constructed, the shortest of them containing only part of the ompC signal sequence and the largest approximately 90% of the sequence encoding mature OmpC protein. Export of the hybrid proteins to a non-cytoplasmic location was a prerequisite for… CONTINUE READING

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