Analysis of Oligonucleotides by HPLC-Electrospray Ionization Mass Spectrometry.


A new interface procedure has been developed that allows, for the first time, the high-efficiency analysis of synthetic oligonucleotides up to 75 bases by reversed-phase HPLC and on-line electrospray ionization mass spectrometry. For oligonucleotides up to 30 bases in length, single-base resolution can be obtained with low levels of cation adduct formation… (More)
DOI: 10.1021/ac960916h


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