Analysis of Ipl1-mediated phosphorylation of the Ndc80 kinetochore protein in Saccharomyces cerevisiae.

@article{Akiyoshi2009AnalysisOI,
  title={Analysis of Ipl1-mediated phosphorylation of the Ndc80 kinetochore protein in Saccharomyces cerevisiae.},
  author={Bungo Akiyoshi and Christian R. Nelson and Jeffrey A Ranish and Sue Biggins},
  journal={Genetics},
  year={2009},
  volume={183 4},
  pages={1591-5}
}
Phosphorylation of the Ndc80 kinetochore protein by the Ipl1/Aurora B kinase reduces its microtubule binding activity in vitro. We found that kinetochore-bound Ndc80 is phosphorylated on Ipl1 sites in vivo, but this phosphorylation is not essential. Instead, we show that additional Ipl1 targets contribute to segregation and the spindle checkpoint.