Analysis of High-Molecular-Weight Ribonucleic Acid Associated with Intracisternal A Particles

@article{Yang1973AnalysisOH,
  title={Analysis of High-Molecular-Weight Ribonucleic Acid Associated with Intracisternal A Particles},
  author={S S Yang and Nelson A. Wivel},
  journal={Journal of Virology},
  year={1973},
  volume={11},
  pages={287 - 298}
}
Intracisternal A particles, known primarily for their association with various tumors, have been shown to contain high-molecular-weight (HMW) ribonucleic acid (RNA) by velocity centrifugation, using linear glycerol gradients. This HMW RNA is sensitive to ribonuclease digestion and alkali treatment but is resistant to Pronase treatment. By a double-labeling experiment, HMW RNA was shown to be intrinsic to intracisternal A particles and not to have resulted from cytoplasmic polysomal RNA… 
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RNA from the two sources directed the synthesis of multiple polypeptides with similar distributions of electrophoretic mobilities in sodium dodecyl sulfate-containing polyacrylamide gels, including one conponent of the same size as the major A-particle structural protein (73,000 daltons).
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TLDR
The RNA isolated from purified A particles is generally of low molecular weight but contains small amount of 70S and 35S components, which suggest that A-type particles may be related to C-type oncornaviruses.
Properties of Intracytoplasmic A Particles Isolated from Oncornavirus-Producing Human Cells
TLDR
A particles isolated from the cytoplasm of HEp-2, HeLa, and AO cells producing oncornavirus of Mason-Pfizer-like type contained neither homological RNA sequences nor common antigens, suggesting that A particles are not intracellular precursors ofason-pfizers-like on cornavirus but represent an independent oncornvirus.
Purification and characterization of DNA polymerases from the plasmacytoma MOPC 104E and Abelson murine leukemia viruses.
RNA-dependent DNA polymerases of intracisternal A particles from the mouse plasma cell tumor MOPC 104E and of Abelson murine leukemia virus (A-MuLV) were isolated from particle preparations by
Properties of an intracisternal A-particle-associated endonuclease activity which is stimulated by ATP
TLDR
An endonuclease activity associated with purified proteinase K-treated intracisternal A-particles was identified and characterized and was greatly stimulated by ATP, ADP, and dATP, whereas AMP appeared to produce a slight inhibition and GTP had no effect on the activity.
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