Analysis of Her2/neu membrane protein clusters in different types of breast cancer cells using localization microscopy

  title={Analysis of Her2/neu membrane protein clusters in different types of breast cancer cells using localization microscopy},
  author={Rainer Kaufmann and Patrick M{\"u}ller and Georg Hildenbrand and M. Hausmann and Christoph Cremer},
  journal={Journal of Microscopy},
The Her2/neu tyrosine kinase receptor is a member of the epidermal growth factor family. It plays an important role in tumour genesis of certain types of breast cancer and its overexpression correlates with distinct diagnostic and therapeutic decisions. Nevertheless, it is still under intense investigation to improve diagnostic outcome and therapy control. In this content, we applied spectral precision distance/position determination microscopy, a technique based on the general principles of… 
Clustered localization of EGFRvIII in glioblastoma cells as detected by high precision localization microscopy.
Cluster analysis revealed that EGFRvIII is present in clusters on the surface of the cells, with about 60% or even more receptor molecules being assembled in clusters of approximately 100 nm in diameter, which will help to unveil the function of this tumour associated receptor variant which might lead to a better understanding of glioblastoma and therefore could lead to improved therapy approaches.
Single molecule localization microscopy coupled with touch preparation for the quantification of trastuzumab-bound HER2
All breast cancers are assessed for levels of human epidermal growth factor receptor 2 (HER2). Fluorescence in situ hybridization (FISH) and immunohistochemistry are currently used to determine if a
Preparation of Immunotoxin Herceptin-Botulinum and Killing Effects on Two Breast Cancer Cell Lines.
Toxin-Herceptin bioconjugation can be a potential candidate with increased efficiency for treating breast cancer patients with over expression of the HER2 receptor.
Quantification of differential ErbB1 and ErbB2 cell surface expression and spatial nanoclustering through plasmon coupling.
It is demonstrated through combination of scattering spectroscopy, electron microscopy, and generalized multiple particle Mie theory (GMT) simulations that the density- and morphology-dependent spectral response of Au nanoparticle (NP) immunolabels bound to the epidermal growth factor receptors ErbB1 andErbB2 encodes quantitative information of both the cell surface expression and spatial clustering of the two receptors in different unliganded in vitro cancer cell lines.
Up-regulation of AKAP13 and MAGT1 on cytoplasmic membrane in progressive hepatocellular carcinoma: a novel target for prognosis.
Evaluation of MAGT1 and AKAP13 expression in clinical HCC tissues by immunohistochemistry suggested that both proteins were strongly expressed in tumor tissues with significantly higher average immunoreactive scores of Remmele and Stegner (IRS) than in non-tumor tissues.
Single-Molecule Localization Microscopy allows for the analysis of cancer metastasis-specific miRNA distribution on the nanoscale
It is concluded that miRs are not only aberrantly expressed and regulated, but also differentially compartmentalized in cells with different metastatic potential and has far reaching potential in defining metastasis-critical subpopulations within a given heterogeneous cancer cell population.
An Evaluation of HER2-Positive Ovarian Carcinoma Xenografts: From a Novel Therapy to a Noninvasive Monitoring Method.
The data demonstrate that the growth of xenografts of human ovarian cancer with high expression of HER2 could be inhibited effectively by Ad-HER2-siRNA+DDP.
Prevalence of HER-2/ neu receptor amplification and its effects over prognosis of the patients with breast cancer
Evaluating the frequency of HER2 (human epidermal growth factor receptor) amplification and its effects over prognosis among the patients with breast cancer reveals 40% patients had HER-2/neu positive expression and was associated with poor outcomes and disease free survival time period in comparison to patient with HER- 2/ neu negative.
Mechanisms of Action of EGFR Tyrosine Kinase Receptor Incorporated in Extracellular Vesicles
Key findings and areas that remain obscure at the intersection of EGFR signalling and EV action are highlighted to cross-fertilise the two fields and speed up the application of novel techniques and paradigms to both.


Activation-dependent clustering of the erbB2 receptor tyrosine kinase detected by scanning near-field optical microscopy.
An increase in cluster size may constitute a general phenomenon in the activation of erbB2, a member of the epidermal growth factor tyrosine kinase family that is often overexpressed in breast cancer and other malignancies.
Studies of the HER-2/neu proto-oncogene in human breast and ovarian cancer.
The concept that the HER-2/neu gene may be involved in the pathogenesis of some human cancers, including breast and ovarian cancer, is supported.
Overexpression of HER-2/neu is associated with poor survival in advanced epithelial ovarian cancer.
Patients whose tumors had high HER-2/neu expression were significantly less likely to have a complete response to primary therapy or have a negative second-look laparotomy when serum CA 125 levels were normal preoperatively.
Human breast cancer: correlation of relapse and survival with amplification of the HER-2/neu oncogene.
Amplification of the HER-2/neu gene was a significant predictor of both overall survival and time to relapse in patients with breast cancer, and had greater prognostic value than most currently used prognostic factors in lymph node-positive disease.
AKT2 is frequently upregulated in HER-2/neu-positive breast cancers and may contribute to tumor aggressiveness by enhancing cell survival
It is suggested that AKT signaling augments resistance to stress-induced apoptosis in breast cancer cells overexpressing HER-2/neu, suggesting a link between AKT activation and stress resistance in HER- 2/neU-overexpressing cells.
Mapping ErbB receptors on breast cancer cell membranes during signal transduction
Moderate co-clustering of ErbB2 and EGFR, even after EGF treatment, suggests that both are predominantly involved in homointeractions.
Detection and Quantification of Small Numbers of Circulating Tumour Cells in Peripheral Blood Using Laser Scanning Cytometer (LSC®)
This method allows quantitation of tumour cells in peripheral blood and bone marrow in reasonable time and will, for the first time, enable extensive investigation of the seeding behaviour of tumours.
Standardized quantification of circulating peripheral tumor cells from lung and breast cancer
The proposed simple and easy method allows close monitoring, which may help in real-time analysis of the response of solid tumors, especially their systemic component, to therapy and hopefully will contribute to more individually tailored therapy.
The ErbB Receptors as Targets for Breast Cancer Therapy
A series of monoclonalantibodies directed against the EGF(ErbB1) receptor and the closely related HER2/Neu( ErbB2) receptor are currently under evaluation, and compounds that inhibit receptortyrosine kinases have shown significant preclinicalactivity and are currently being evaluated in the clinic.
Untangling the ErbB signalling network
When epidermal growth factor and its relatives bind the ErbB family of receptors, they trigger a rich network of signalling pathways, culminating in responses ranging from cell division to death,