• DOI:10.1038/nprot.2013.095
Corpus ID: 22645510

Analysis of GSH and GSSG after derivatization with N-ethylmaleimide

@article{Giustarini2013AnalysisOG,
  title={Analysis of GSH and GSSG after derivatization with N-ethylmaleimide},
  author={D. Giustarini and I. Dalle-donne and A. Milzani and P. Fanti and R. Rossi},
  journal={Nature Protocols},
  year={2013},
  volume={8},
  pages={1660-1669}
}

D. Giustarini, I. Dalle-donne, +2 authors R. Rossi

Published 2013

Chemistry, Medicine

Nature Protocols

This protocol describes a procedure for determining glutathione (GSH) and glutathione disulfide (GSSG) concentrations in blood and other tissues. Artifactual oxidation to GSSG of 5–15% of the GSH found in a sample can occur during deproteination of biological samples with any of the commonly used acids, with consequent marked overestimation of GSSG. This can be prevented by derivatizing GSH with the alkylating agent N-ethylmaleimide (NEM) to form GS-NEM before acid deproteination, followed by… CONTINUE READING

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