Analysis of Extracellular Proteins of Aspergillus oryzae Grown on Soy Sauce Koji
@article{Liang2009AnalysisOE, title={Analysis of Extracellular Proteins of Aspergillus oryzae Grown on Soy Sauce Koji}, author={Y Liang and Li Pan and Ying-Wu Lin}, journal={Bioscience, Biotechnology, and Biochemistry}, year={2009}, volume={73}, pages={192 - 195} }
Aspergillus oryzae AS 3.951 is widely used in Chinese soy sauce manufacture, but little is known about the profiles of the extracellular proteins from the culture of soybean koji. In this study, we carried out MALDI-TOF/TOF MS analysis of extracellular proteins during koji culture. Besides well-known proteins (TAA and Oryzin), a variety of aminopeptidase and proteases were identical at the proteome level. This suggests that A. oryzae AS 3.951 has a powerful capacity to digest soybean protein.
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References
SHOWING 1-10 OF 16 REFERENCES
Identification of secreted proteins of Aspergillus oryzae associated with growth on solid cereal substrates.
- Biology, MedicineJournal of biotechnology
- 2006
Genomics of Aspergillus oryzae
- Biology, EngineeringBioscience, biotechnology, and biochemistry
- 2007
The genome sequence of Aspergillus oryzae, a fungus used in the production of the traditional Japanese fermentation foods sake (rice wine), shoyu (soy sauce), and miso (soybean paste), has revealed…
Proteomic analysis of rutin-induced secreted proteins from Aspergillus flavus.
- BiologyFungal genetics and biology : FG & B
- 2004
Proteomic Analysis of Extracellular Proteins from Aspergillus oryzae Grown under Submerged and Solid-State Culture Conditions
- BiologyApplied and Environmental Microbiology
- 2006
A comparative proteome analysis of extracellular proteins in solid-state and submerged (liquid) cultures suggested that the secretion of TAA and Bgl was regulated by trapping these proteins in the cell wall in submerged culture and that secretion of GlaA and XynG2 was regulated at the posttranscriptional level in the solid- state culture.
The state of proteome profiling in the fungal genus Aspergillus.
- Biology, EngineeringBriefings in functional genomics & proteomics
- 2008
A summary proteome map highlighting identified proteins in major metabolic pathway is presented and a combined total of 28 cell surface, 102 secreted and 139 intracellular proteins have been identified based on 10 different studies on Aspergillus proteomics.
[Isolation and properties of leucine aminopeptidase from Aspergillus oryzae].
- BiologyBiokhimiia
- 1977
Homogenious leucine aminopeptidase is purified from "oryzine"--mixture of enzymes produced by surface culture of Asperigillus oryzae using treatment with activated characoal, followed by…
Analysis of secreted proteins from Aspergillus flavus
- BiologyProteomics
- 2005
This is the first extensive proteomic study conducted to identify the secreted proteins from a filamentous fungus, Aspergillus flavus, when grown on rutin and glucose and potato dextrose.
Genome sequencing and analysis of Aspergillus oryzae
- BiologyNature
- 2005
Specific expansion of genes for secretory hydrolytic enzymes, amino acid metabolism and amino acid/sugar uptake transporters supports the idea that A. oryzae is an ideal microorganism for fermentation.
Protein secretion in filamentous fungi--trying to understand a highly productive black box.
- BiologyTrends in biotechnology
- 1994
Transcriptional analysis of genes for energy catabolism and hydrolytic enzymes in the filamentous fungus Aspergillus oryzae using cDNA microarrays and expressed sequence tags
- BiologyApplied Microbiology and Biotechnology
- 2004
The wheat-bran culture gave the richest gene expression profile of hydrolytic enzymes and the lowest expression levels of catabolic genes (EMP, TCA) among the three media tested, suggesting that A. oryzae uses both EMP and TCA for glucose metabolism under AC conditions.