Analysis of E. coli K5 capsular polysaccharide heparosan.

  title={Analysis of E. coli K5 capsular polysaccharide heparosan.},
  author={Mellisa Ly and Zhenyu Wang and Tatiana N. Laremore and Fuming Zhang and Wei-hong Zhong and Dennis Pu and Dmitri V Zagorevski and Jonathan S. Dordick and Robert J. Linhardt},
  journal={Analytical and bioanalytical chemistry},
  volume={399 2},
Heparosan is the key precursor for the preparation of bioengineered heparin, a potential replacement for porcine intestinal heparin, an important anticoagulant drug. The molecular weight (MW) distribution of heparosan produced by the fermentation of E. coli K5 was investigated. Large-slab isocratic and mini-slab gradient polyacrylamide gel electrophoresis (PAGE) were used to analyze the MW and polydispersity of heparosan. A preparative method that allowed fractionation by continuous-elution… CONTINUE READING
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