Analysis of Dynactin Subcomplexes Reveals a Novel Actin-Related Protein Associated with the Arp1 Minifilament Pointed End

@article{Eckley1999AnalysisOD,
  title={Analysis of Dynactin Subcomplexes Reveals a Novel Actin-Related Protein Associated with the Arp1 Minifilament Pointed End},
  author={D. Mark Eckley and Steven R. Gill and Karin A. Melkonian and James B. Bingham and Holly V. Goodson and John E. Heuser and Trina A Schroer},
  journal={The Journal of Cell Biology},
  year={1999},
  volume={147},
  pages={307 - 320}
}
The multisubunit protein, dynactin, is a critical component of the cytoplasmic dynein motor machinery. Dynactin contains two distinct structural domains: a projecting sidearm that interacts with dynein and an actin-like minifilament backbone that is thought to bind cargo. Here, we use biochemical, ultrastructural, and molecular cloning techniques to obtain a comprehensive picture of dynactin composition and structure. Treatment of purified dynactin with recombinant dynamitin yields two… CONTINUE READING
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