Analysis of DNA repeats in bacterial plasmids reveals the potential for recurrent instability events

  title={Analysis of DNA repeats in bacterial plasmids reveals the potential for recurrent instability events},
  author={Pedro H. Oliveira and Kristala Jones Prather and Duarte Miguel F. Prazeres and Gabriel A. Monteiro},
  journal={Applied Microbiology and Biotechnology},
Structural instability has been frequently observed in natural plasmids and vectors used for protein expression or DNA vaccine development. However, there is a lack of information concerning hotspot mapping, namely, DNA repeats or sequences identical to the host genome. This led us to evaluate the abundance and distribution of direct, inverted, and tandem repeats with high recombination potential in 36 natural plasmids from ten bacterial genera, as well as in several widely used bacterial and… 

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