Analysis of DNA repair pathways of Schizosaccharomyces pombe by means of swi-rad double mutants.

@article{Schlake1993AnalysisOD,
  title={Analysis of DNA repair pathways of Schizosaccharomyces pombe by means of swi-rad double mutants.},
  author={C Schlake and Kai Ostermann and Henning Schmidt and Herbert Gutz},
  journal={Mutation research},
  year={1993},
  volume={294 1},
  pages={
          59-67
        }
}
The rad16 gene of Schizosaccharomyces pombe: a homolog of the RAD1 gene of Saccharomyces cerevisiae
TLDR
Two-hybrid experiments indicate a strong protein-protein interaction between the products of the rad16 and swi10 genes of S. pombe, which mirrors that reported for RAD1 and RAD10 in S. cerevisiae.
Involvement of Schizosaccharomyces pombe Srs2 in cellular responses to DNA damage.
TLDR
It is concluded that Srs2 plays related, but not identical, roles in the two yeast species.
Compensatory role for Rad52 during recombinational repair in Ustilago maydis
TLDR
The results suggest that Rad52 serves in an overlapping, compensatory role with both Rec2 and Brh2 to promote and maintain formation of the Rad51 nucleoprotein filament.
Rad22 Protein, a Rad52 Homologue inSchizosaccharomyces pombe, Binds to DNA Double-strand Breaks*
TLDR
It is shown that Rad22 protein, a Rad52 homologue in the fission yeast Schizosaccharomyces pombe, can similarly bind to DNA ends at double-strand breaks, the first evidence that a recombinational protein directly binds to DNA double-Strand breaks in vivo.
A double-strand break repair component is essential for S phase completion in fission yeast cell cycling.
TLDR
A cell division cycle mutant with a temperature-sensitive allele of rad22(+), designated rad22-H6, which resulted from a point mutation in the conserved coding sequence leading to one amino acid alteration is obtained.
The genetics of the repair of 5-azacytidine-mediated DNA damage in the fission yeastSchizosaccharomyces pombe
TLDR
Using a combination of single-mutant and epistasis analyses, it is found that the excision, mismatch and recombinational repair pathways are all required in some degree for the repair of 5-azaC-mediated DNA damage.
A fission yeast homolog of CDC20/p55CDC/Fizzy is required for recovery from DNA damage and genetically interacts with p34cdc2
TLDR
Molecular cloning of slp1+ revealed that slp 1+ encodes a putative 488-amino-acid polypeptide exhibiting significant homology to WD-domain proteins, namely, CDC20 (budding yeast), p55CDC (human), and Fizzy (fly), which may be involved in a critical process which restarts cell cycle progression after the completion of DNA repair.
An alternative eukaryotic DNA excision repair pathway
TLDR
It is proposed that the SPDE-dependent DNA repair pathway is the second DNA excision repair process present in S. pombe and the resulting double mutant was much more sensitive to UV radiation than either single mutant.
Yeast mutants as a model system for identification of determinants of chemosensitivity.
TLDR
The role of genes involved in repair processes and in checkpoint control are outlined, with specific reference to genes regulating radiation-sensitivity, and this fast-moving field shows promise as a novel and potentially useful method for development of target-specific therapeutic approaches.
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References

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The rad3+ gene of Schizosaccharomyces pombe is involved in multiple checkpoint functions and in DNA repair.
TLDR
This work has found that the radiation-sensitive Schizosaccharomyces pombe mutant rad3-136 is deficient in two molecular checkpoint functions and has suggested that the rad3+ gene is likely to play a role in DNA repair.
DNA repair mutants defining G2 checkpoint pathways in Schizosaccharomyces pombe.
TLDR
The phenotype of these checkpoint rad mutants in wee mutant backgrounds indicate that the G2 arrest response is mediated either through, or in parallel with, the activity of the cdc2 gene product, which suggests that the pathway responsible for the recognition of DNA damage and the subsequent mitotic arrest, shares many functions with the mechanism that controls the dependency of mitosis on the completion of S phase.
Checkpoint controls in Schizosaccharomyces pombe: rad1.
TLDR
The rad series of radiosensitive fission yeast mutants was examined to identify strains deficient for the DNA damage‐responsive checkpoint control, and rad1‐1 was implicated in this role by the coincidence of the radiation transition point and the cdc2 execution point.
Genetic control of radiation sensitivity in Schizosaccharomyces pombe.
TLDR
Genetic analysis of a large number of radiation-sensitive mutants of S. pombe, isolated in different laboratories, showed that these isolates represent 22 non-allelic loci, which implies the presence of multiple pathways in S.pombe for repair of Radiation-induced damage.
The protein sequence and some intron positions are conserved between the switching gene swi10 of Schizosaccharomyces pombe and the human excision repair gene ERCC1.
TLDR
Northern blot experiments and the analyses of cDNA clones indicate that intron I of the swi10 gene is not efficiently spliced, and the Swi10 protein contains a putative DNA binding domain previously found in other proteins.
Incision and postincision steps of pyrimidine dimer removal in excision-defective mutants of Saccharomyces cerevisiae
TLDR
In strains containing excision-defective mutations in any of nine genes in combination with the cdc9 mutation, the absence ofLow-molecular-weight DNA at the nonpermissive temperature after ultraviolet treatment suggests that these mutants are incision defective, whereas the presence of low-molescular- Weight DNA indicates that the mutants are defective in a step after incision.
Radiation resistance in Schizosaccharomyces pombe
TLDR
The advantages of S. pombe are outlined and the radiation‐sensitive mutants available for the analysis of DNA repair and recombination mechanisms in this organism are described.
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