Analysis of DNA processing reactions in bacterial conjugation by using suicide oligonucleotides.

@article{GonzlezPrez2007AnalysisOD,
  title={Analysis of DNA processing reactions in bacterial conjugation by using suicide oligonucleotides.},
  author={Blanca Gonz{\'a}lez-P{\'e}rez and Mar{\'i}a Lucas and Leonie A Cooke and Joseph S. Vyle and F Sebasti{\'a}n de la Cruz and Gabriel Moncali{\'a}n},
  journal={The EMBO journal},
  year={2007},
  volume={26 16},
  pages={
          3847-57
        }
}
Protein TrwC is the conjugative relaxase responsible for DNA processing in plasmid R388 bacterial conjugation. TrwC has two catalytic tyrosines, Y18 and Y26, both able to carry out cleavage reactions using unmodified oligonucleotide substrates. Suicide substrates containing a 3'-S-phosphorothiolate linkage at the cleavage site displaced TrwC reaction towards covalent adducts and thereby enabled intermediate steps in relaxase reactions to be investigated. Two distinct covalent TrwC… CONTINUE READING
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